MO-MAGE method

MO-MAGE method for targeted whole-genome mutagenesis; 130 base oligonucleotides were designed and synthesized on a biochip. Oligos are amplified by the polymerase chain reaction (PCR) technique and enzymatically treated into form for MO-MAGE. On the left: MO-MAGE of 2,587 genomic targets corresponding to untranslated regions (UTR) upstream of genes for insertion of 20-bp T7 synthetic promoter. Designed targets are shown in blue. Mutated targets verified by whole-genome sequencing are shown in red. Mutated targets verified by amplicon sequencing are shown in black.